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Local and systemic cytokine expression during experimental chronic Trypanosoma cruzi infection in a Cebus monkey model

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dc.contributor.author Rojas de Arias, Antonieta
dc.contributor.author Samudio, Margarita
dc.contributor.author Montenegro-James, Sonia
dc.contributor.author Kasamatsu, Elena
dc.contributor.author Cabral, Margarita
dc.contributor.author Schinini, Alicia
dc.contributor.author James, Mark A.
dc.date.accessioned 2017-01-07T20:53:34Z
dc.date.available 2017-01-07T20:53:34Z
dc.date.issued 1999
dc.identifier.uri http://localhost:8080/xmlui-directorioacademico/handle/123456789/60
dc.description.abstract Cebus apella is an acceptable model for chronic chagasic cardiomyopathy (CCC), since it is possible to experimentally induce cardiac lesions after 1 year of Trypanosoma cruzi infection. The T. cruzi Y strain, shown previously to produce CCC in C. apella monkeys, was used to experimentally infect 10 monkeys. Parasitological, serological and clinical parameters were monitored during a 19-month follow-up, and systemic cytokine responses were assessed sequentially in five monkeys selected according to the differential parasitemia pattern exhibited. Ten additional monkeys, infected with the same strain for 5, 10 and 12 years, were analysed cross-sectionally. Three monkeys/time point and one uninfected control animal were sacrificed for gross pathology, histology, presence of parasites, and local cytokine gene expression. Elevated expression of interleukin (IL)-4 was observed throughout the study in monkeys that had persistent, high parasitemias, whereas a high level of interferon (IFN)-gamma was seen in monkeys that controlled parasitemias soon after infection. Chronically infected monkeys expressed a nonpolarized, Th0-type response. Cardiac tissue collected from a monkey that succumbed to acute infection had elevated levels of proinflammatory cytokine [IL-1beta, IL-6, tumour necrosis factor-alpha] and interstitial cell adhesion molecule (ICAM)-1, platelet-derived growth factor (PDGF)-alpha, transforming growth factor (TGF)-beta and IL-10 transcripts. Cytokine production in cardiac tissue of chronically infected monkeys was also characterized by elevated expression of ICAM-1, PDGF-alpha and TGF-beta, which correlated with the detection of T. cruzi DNA by polymerase chain reaction.
dc.format application/pdf
dc.source Parasite Inmunolgy
dc.subject Trypanosoma cruzi
dc.subject Chagas’ disease
dc.subject Cebus apella
dc.subject RT-PCR
dc.subject cytokines
dc.subject RT-PCR
dc.subject.mesh Acute Disease
dc.subject.mesh Animals
dc.subject.mesh Cebus
dc.subject.mesh Chagas Cardiomyopathy/genetics
dc.subject.mesh Chagas Cardiomyopathy/immunology
dc.subject.mesh Chagas Cardiomyopathy/parasitology
dc.subject.mesh Chagas Cardiomyopathy/pathology
dc.subject.mesh Chronic Disease
dc.subject.mesh Cytokines/analysis
dc.subject.mesh Cytokines/blood
dc.subject.mesh Cytokines/genetics
dc.subject.mesh Cytokines/immunology
dc.subject.mesh Disease Models, Animal
dc.subject.mesh Electrocardiography
dc.subject.mesh Female
dc.subject.mesh Intercellular Adhesion Molecule-1/genetics
dc.subject.mesh Interferon-gamma/analysis
dc.subject.mesh Interferon-gamma/blood
dc.subject.mesh Interferon-gamma/genetics
dc.subject.mesh Interferon-gamma/immunology
dc.subject.mesh Interleukins/analysis
dc.subject.mesh Interleukins/blood
dc.subject.mesh Interleukins/genetics
dc.subject.mesh Interleukins/immunology
dc.subject.mesh Male
dc.subject.mesh Myocardium/immunology
dc.title Local and systemic cytokine expression during experimental chronic Trypanosoma cruzi infection in a Cebus monkey model
dc.type Artículo
dcterms.bibliographicCitation Parasite Inmunolgy 21(9), 451 - 460. 1999
dc.source.volume 21
dc.source.number 9
dc.source.pages 451 - 460
dc.contributor.identifier 1261551
dc.contributor.identifier 998549
dc.contributor.identifier 232518
dc.contributor.identifier 600442
dc.contributor.identifier 294795
dc.directorio.index MEDLINE

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