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Trypanosoma cruzi: Polymerase Chain Reaction-Based Detection in Dried Feces of Triatoma infestans

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dc.contributor.author Rojas de Arias, Antonieta
dc.contributor.author Russomando, Graciela
dc.contributor.author Almiron, María
dc.contributor.author Figueredo, Antonio
dc.contributor.author Ferreira, María Elena
dc.contributor.author Morita, Kouichi
dc.date.accessioned 2017-01-07T20:53:28Z
dc.date.available 2017-01-07T20:53:28Z
dc.date.issued 1996
dc.identifier.uri http://localhost:8080/xmlui-directorioacademico/handle/123456789/33
dc.description.abstract PCR was employed to detect Trypanosoma cruzi DNA in Triatoma infestans dry fecal spots collected on filter papers. Both insects fed on experimentally infected monkeys and insects collected in a Paraguayan endemic area for Chagas' disease were examined. When the insects fed on a chronically infected monkey with low parasitemia as revealed by direct microscopic observation (DMO), T. cruzi was detected in the insect feces by PCR as soon as 2 days postfeeding. When the same experiment was performed on monkeys with parasitemia levels below the limit of detection by DMO, the degree of positivity found through PCR-Southern hybridization, applied on Day 8 postfeeding, was superior to that obtained through xenoculture. These results suggest that PCR can be used to speed the xenodiagnosis results with great sensitivity. On the other hand, when applied to the feces of triatomines collected in the field, 84% were positive by PCR-Southern hybridization, whereas only 26% were positive by DMO. Therefore, PCR could also be applied to the monitoring of the infection status of triatomines which infest rural dwellings by examining only the feces left on paper sensors hung on the walls of the houses.
dc.format application/pdf
dc.rights Academic Press, Inc.
dc.source Phytotherapy Research
dc.subject Trypanosoma cruzi
dc.subject Triatoma infestans
dc.subject triatomine
dc.subject xenodiagnosis
dc.subject PCR, polymerase chain reaction
dc.subject DMO, direct microscopic observation
dc.subject.mesh Animals
dc.subject.mesh Blotting, Southern
dc.subject.mesh Cebus
dc.subject.mesh Chagas Disease/diagnosis
dc.subject.mesh Chagas Disease/parasitology
dc.subject.mesh DNA, Protozoan/analysis
dc.title Trypanosoma cruzi: Polymerase Chain Reaction-Based Detection in Dried Feces of Triatoma infestans
dc.type Artículo
dcterms.bibliographicCitation Phytotherapy Research 83(1), 62 - 66. 1996
dc.source.volume 83
dc.source.number 1
dc.source.pages 62 - 66
dc.contributor.identifier 1261551
dc.contributor.identifier 608027
dc.contributor.identifier 517692
dc.identifier.doi 10.1006/expr.1996.0049


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